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On-site classification of pansteatitis in Mozambique tilapia (oreochromis mossambicus) using a portable Lipid-Based analyser

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dc.contributor.author Bowden, JA
dc.contributor.author Somerville, SE
dc.contributor.author Cantu, TM
dc.contributor.author Guillette, MP
dc.contributor.author Botha, H
dc.contributor.author Boggs, ASP
dc.contributor.author Luus-Powell, W
dc.contributor.author Guillette, LJ. Jr
dc.date.accessioned 2018-08-07T11:24:37Z
dc.date.available 2018-08-07T11:24:37Z
dc.date.copyright 2016-05-16
dc.date.issued 2016-05-16
dc.identifier.citation Bowden, JA, Somerville, SE, Cantu, TM, Guillette, MP, Botha, H, Boggs, ASP et al. 2016, ‘On-site classification of pansteatitis in Mozambique tilapia (oreochromis mossambicus) using a portable lipid-based analyzer’, Analytical Methods, Vol. 36, No. 7, pp. 6631–6635, Viewed 07 August 2018, Royal Society of Chemistry, doi:10.1039/C6AY00446F en_US
dc.identifier.issn 1759-9660
dc.identifier.uri https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5514565/
dc.identifier.uri http://hdl.handle.net/10907/2730
dc.description.abstract While no pansteatitis-related large-scale mortality events have occurred since 2008, the current status of pansteatitis (presence and pervasiveness) in the Olifants River system and other regions of South Africa remain largely unknown. In part, this is due to both a lack of known biological markers of pansteatitis and a lack of suitable non-invasive assays capable of rapidly classifying the disease. Here, we propose the application of a point-of-care (POC) device using lipid-based test strips (total cholesterol (TC) and total triglyceride (TG)), for classifying pansteatitis status in the whole blood of pre-spawning Mozambique tilapia (Oreochromis mossambicus). Using the TC strips, the POC device was able to non-lethally classify the tilapia as either healthy or pansteatitis-affected; the sexes were examined independently because sexual dimorphism was observed for TC (males p = 0.0364, females χ2 = 0.0007). No significant difference between diseased and pansteatitis-affected tilapia was observed using the TG strips. This is one of the first described applications of using POC devices for on-site environmental disease state testing. A discussion on the merits of using portable lipid-based analyzers as an in-field disease-state diagnostic tool is provided. en_US
dc.description.sponsorship National Research Foundation (South Africa) en_US
dc.format.extent Tables:1 , fig: 2 , 11p en_US
dc.format.medium PDF en_US
dc.language.iso en en_US
dc.publisher Royal Society of Chemistry en_US
dc.rights Attribution-NonCommercial-NoDerivs 3.0 United States en_US
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/us/ en_US
dc.subject Cholesterol en_US
dc.subject Pansteatitis en_US
dc.subject Triglyceride en_US
dc.subject Mozambique Tilapia en_US
dc.title On-site classification of pansteatitis in Mozambique tilapia (oreochromis mossambicus) using a portable Lipid-Based analyser en_US
dc.type Article en_US
dc.rights.holder Royal Society of Chemistry en_US


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