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Classification of ‘Granny Smith’ apples with different levels of superficial scald severity based on targeted metabolites and discriminant analysis

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dc.contributor.author Mditshwa, A
dc.contributor.author Fawole, OA
dc.contributor.author Vries, F
dc.contributor.author van der Merwe, K
dc.contributor.author Crouch, E
dc.contributor.author Opara, UL
dc.date.accessioned 2018-09-14T09:56:42Z
dc.date.available 2018-09-14T09:56:42Z
dc.date.copyright 2016-02-29
dc.date.issued 2016-02-29
dc.identifier.citation Mditshwa, A , Fawole, OA, Vries, F, van der Merwe, K , Crouch, E & Opara, UL 2016, ‘Classification of ‘Granny Smith’ apples with different levels of superficial scald severity based on targeted metabolites and discriminant analysis’, Journal of Applied Botany and Food Quality, vol. 89, Viewed 04 September 2018, Julius Kühn-Institut - Bundesforschungsinstitut für Kulturpflanzen, https://doi.org/10.5073/JABFQ.2016.089.006 en_US
dc.identifier.issn 1613-9216
dc.identifier.uri https://ojs.openagrar.de/index.php/JABFQ/article/view/4589
dc.identifier.uri http://hdl.handle.net/10907/2967
dc.description.abstract To study the metabolic changes in ‘Granny Smith’ apples with different severities of superficial scald, fruit were stored in normal refrigerated air (0°C, 95% RH) for 12 weeks followed by 7 d shelf-life under room conditions (20°C, 65% RH). Fruit were graded to five groups based on scald severity and analysed for ethylene, α-farnesene and 6-methyl-5-hepten-2-one (MHO) levels. Reactive oxygen species (ROS) were measured by confocal laser-scanning microscopy on apple peel treated with fluorescent probe 2’,7’-dichlorodihydrofluorescein diacetate. Ethylene production rate, α-farnesene and MHO contents and ROS intensity increased with increasing scald severity but declined in severely scalded fruit. Malondialdehyde (MDA) concentration in fruit peel, a measure of membrane damage, increased linearly (R=0.891) with increase in scald severity. Discriminant analysis was used to classify fruit by scald severity on the basis of metabolites accumulated. The stepwise model indicated that three attributes (ROS, ethylene production and MDA) contributed significantly (R2≥0.5) to the separation of the five scald severity indexes, with ROS having the highest contribution (partial R² =0.961; p<0.0001), followed by ethylene (R2=0.718; p<0.0001) and MDA (R2=0.578; p<0.0001). en_US
dc.description.sponsorship National Research Foundation (South Africa) en_US
dc.format.extent 7 p. en_US
dc.format.medium PDF en_US
dc.language.iso en en_US
dc.publisher Julius Kühn-Institut - Bundesforschungsinstitut für Kulturpflanzen en_US
dc.rights Attribution-NonCommercial-NoDerivs 3.0 United States en_US
dc.subject Apples en_US
dc.subject Scald Severity en_US
dc.subject Alpha-Farnescence en_US
dc.subject Ethylene, quality en_US
dc.title Classification of ‘Granny Smith’ apples with different levels of superficial scald severity based on targeted metabolites and discriminant analysis en_US
dc.type Article en_US
dc.rights.holder Julius Kühn-Institut - Bundesforschungsinstitut für Kulturpflanzen en_US


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