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Studies on the in vitro time kill assessment of crude acetone and aqueous extracts of Helichrysum pedunculatum leaves

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dc.contributor.author Aiyegoro, A.O.
dc.contributor.author Afolayan, A.J.
dc.contributor.author Okoh, A.I.
dc.date.accessioned 2016-11-21T12:50:43Z
dc.date.available 2016-11-21T12:50:43Z
dc.date.copyright 2008
dc.date.created 2016
dc.date.issued 2008
dc.identifier.citation Aiyegoro A.O ,Afolayan A.J & Okoh A.I 2008 ‘Studies on the in vitro time kill assessment of crude acetone and aqueous extracts of Helichrysum pedunculatum leaves’, African Journal of Biotechnology, Vol. 7, no. 20, pp. 3718-3722 en_US
dc.identifier.issn 1684–5315
dc.identifier.uri http://hdl.handle.net/10907/833
dc.description.abstract Helichrysum pedunculatum is used in folklore remedies to dress wound acquired after circumcision rite. This led to the study of in vitro antibacterial activities of this medicinal plant. Using standard microbiological procedures, six bacteria species made up of four gram positive and two gram negative bacteria were screened for susceptibility to crude acetone and aqueous extracts of this plant. The minimum inhibitory concentrations (MICs) of the acetone extract against the susceptible bacteria was 5.0 mg/ml while that of the aqueous extract ranged between 0.5 - 35 mg/ml. Average log reduction in viable cell count in time kill assay of the acetone extract ranged between 0.64 Log10 and 5.99 Log10 cfu/ml after 6 h of interaction, and between 5.99 Log10 and 6.06 Log10 cfu/ml after 12 h interaction in 1 × MIC and 2 × MIC, and between 0.10 Log10 to 0.33 Log10 cfu/ml after 6 h of interaction, and 0.23 Log10 and 0.56 Log10 cfu/ml after 12 h interaction in 1 × MIC and 2 × MIC for the aqueous extract. The effect of the aqueous extract was only bacteriostatic on both reference and environmental strains and the clinical isolates were outrightly resistant to this extract (not reported here). This is worrisome and this could be one reason why, there is an incidence of high death rate resulting from circumcision wounds infection even after treating such wounds with H. pedunculatum leaf. Perhaps the plant could be of more relevance in combination therapy and a source of resistance modifying principles which is the subject of on going studies in our group. en_US
dc.description.sponsorship National Research Foundation (NRF), Ref: TTK2006061400023. en_US
dc.format.extent 5 p. en_US
dc.format.medium PDF en_US
dc.language.iso en en_US
dc.publisher Academic Journals en_US
dc.relation.requires Adobe Acrobat Reader en_US
dc.subject Antibacterial en_US
dc.subject Bactericidal en_US
dc.subject Bacteriostatic en_US
dc.subject Time-kill en_US
dc.subject Log reduction en_US
dc.subject Minimum inhibitory concentration en_US
dc.subject Helichrysum pedunculatum en_US
dc.title Studies on the in vitro time kill assessment of crude acetone and aqueous extracts of Helichrysum pedunculatum leaves en_US
dc.type Article en_US
dc.rights.holder National Research Foundation en_US


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